Ultra-sensitive detection of Mycobacterium leprae: DNA extraction and PCR assays
نویسندگان
چکیده
منابع مشابه
A RAPID AND SIMPLE METHOD OF DNA EXTRACTION FROM CLINICAL SPECIMENS CONTAINING MYCOBACTERIUM LEPRAE FOR PCR ASSAYS
We describe a rapid, simple, and reliable procedure for routine isolation of Mycobacterium leprae DNA from slit-skin swab specimens. This one-step DNA extraction method is based on the utilization of ChelexR 100, a chelating ion exchange resin. Slit-skin swab specimens from seven untreated leprosy patients at Baba Baghi Leprosy Hospital in Iran were processed by this procedure. The polymer...
متن کاملa rapid and simple method of dna extraction from clinical specimens containing mycobacterium leprae for pcr assays
we describe a rapid, simple, and reliable procedure for routine isolation of mycobacterium leprae dna from slit-skin swab specimens. this one-step dna extraction method is based on the utilization of chelexr 100, a chelating ion exchange resin. slit-skin swab specimens from seven untreated leprosy patients at baba baghi leprosy hospital in iran were processed by this procedure. the polymerase c...
متن کاملFTA card utility for PCR detection of Mycobacterium leprae.
The suitability of the FTA® elute card for the collection of slit skin smear (SSS) samples for PCR detection of Mycobacterium leprae was evaluated. A total of 192 SSS leprosy samples, of bacillary index (BI) 1 to 5, were collected from patients attending two skin clinics in Myanmar and preserved using both FTA® elute cards and 70% ethanol tubes. To compare the efficacy of PCR detection of DNA f...
متن کاملReverse transcription-PCR detection of Mycobacterium leprae in clinical specimens.
A reverse transcription (RT)-PCR assay targeting the 16S rRNA of Mycobacterium leprae was developed to detect the organism in clinical specimens. A 171-bp fragment was amplified when M. leprae RNA was used as a template but not when a panel of RNAs from 28 potentially cross-reacting mycobacterial species, seven genera related to Mycobacterium, and three organisms normally found among skin or no...
متن کاملDNA metabolism in Mycobacterium leprae.
Understanding the molecular basis underlying the hallmark features of Mycobacterium leprae, such as its parasitism and extraordinarily slow growth rate, has stimulated research into the biology of this pathogen for decades, but it is through the completion of its genome sequence that a quantum leap of progress has been achieved. 1 ln this review, we analyse the genes in M. leprae that are invol...
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ژورنال
عنوان ژورنال: PLOS Neglected Tropical Diseases
سال: 2020
ISSN: 1935-2735
DOI: 10.1371/journal.pntd.0008325